Tip of the Month - Array Serial Numbers

The format of the serial number

Probably you have noticed that for some time now, all of the ECIS arrays are shipped with a serial number affixed either to the side (96 wells) or near the contact pads (8 wells). The serial number begins with a letter(s) that identifies the type of array followed by a production number that is unique for that specific array only. This is followed by the same information in a bar code (code 128) format such that it can be read by a scanner.

For example below one can see the label on a 96 well array.


The letter B on the 96 well array serial number designates that this is a 96W1E+PET array, a different letter would be used even if the array were made on a polycarbonate substrate instead of PET (polyethylene terephthalate) or if the electrode pattern were not the 1E+ configuration.


The letter E on the eight well array serial number above designates that this is a 8W10E on a PET substrate.

Good laboratory practice

For good laboratory practice, one should always input the array serial number so that it becomes a part of the data file(s) acquired from that array. In this manner, the exact type of array used is recorded, and should one store the array with fixed cells, it can be located and definitively identified as belonging to a specific experiment.

Inputting the serial number

There are different ways to attach the serial number of an array to the data file. One straightforward approach is to simply type the number into the file comment section. There are other means to accomplish this either using a scanner at the start of the experiment where you will be prompted to scan the array or going Acquire tab and selecting Array Serial # in the drop down menu and inputting the serial number.
 
 
 
 
ECIS Application Webinars

ECIS application webinars review the topics listed below in 20 to 30 minute, web-based, interactive seminars presented by Applied BioPhysics President and co-founder, Dr. Charles Keese.

All webinars are held at 11:00 am EST. To register for a webinar, please go to: https://appliedbiophysics.webex.com and scroll to the webinar date of interest.  

ECIS Theory – May 2, 2017

Cell Invasion / Extravasation Assays – May 16, 2017

Automated Cell Migration – June 6, 2017
 
 
 
 
Latest ECIS Software   
 
The latest version of the software is v1.2.215 available from:

 
 
 
 
Tradeshows & Events 

American Thoracic Society 2017
May 19 – 24, 2017
Washington, DC 


2017 ASCB / EMBO
Dec 2 – 6, 2017
Philadelphia, PA
 
 
 
 

ECIS School Graduates - March 2017

From left to right: Ying Liu-University of Pennsylvania, Koreana Pak-University of Pennsylvania, Nektarios Barabutis- Old Dominion University, Raji Rajesh Lenin-University of Tennessee Health Science Center, Bethany Young-Virginia Commonwealth University, Gaelle Mercenne-Recursion Pharmaceuticals, LLC.
 
 
 
 
New Publications

Many new publications have been added to the Applied BioPhysics ECIS publications database.  The following are just a selection of those added.

Arnardottir, Hildur H., Jesmond Dalli, Lucy V. Norling, Romain A. Colas, Mauro Perretti, and Charles N. Serhan. 2016. “Resolvin D3 Is Dysregulated in Arthritis and Reduces Arthritic Inflammation.” The Journal of Immunology 197 (6): 2362–68. doi:10.4049/jimmunol.1502268.

Bartlett, Annie, Andrew Sanders, Fiona Ruge, Keith Harding, and Wen Jiang. 2016. “Potential Implications of Interleukin-7 in Chronic Wound Healing.” Experimental and Therapeutic Medicine, April. doi:10.3892/etm.2016.3263.

Bhargavan, Biju, and Georgette D. Kanmogne. 2017. “Differential Mechanisms of Inflammation and Endothelial Dysfunction by HIV-1 Subtype-B and Recombinant CRF02_AG Tat Proteins on Human Brain Microvascular Endothelial Cells: Implications for Viral Neuropathogenesis.” Molecular Neurobiology, January. doi:10.1007/s12035-017-0382-0.

Bolinger, Mark T., Aniket Ramshekar, Helen V. Waldschmidt, Scott D. Larsen, Maria C. Bewley, John M. Flanagan, and David A. Antonetti. 2016. “Occludin S471 Phosphorylation Contributes to Epithelial Monolayer Maturation.” Molecular and Cellular Biology 36 (15): 2051–66. doi:10.1128/MCB.00053-16.

Cha, Byong-Ki, Young-Suk Kim, Ki-Eun Hwang, Kyung-Hwa Cho, Byoung-Ryun Kim, Hong-Young Jun, Kwon-Ha Yoon, Eun-Taik Jeong, and Hak-Ryul Kim. 2016. “Celecoxib and Sulindac Inhibit TGF-β1-Induced Epithelial-Mesenchymal Transition and Suppress Lung Cancer Migration and Invasion via Downregulation of Sirtuin 1.” Oncotarget, August. doi:10.18632/oncotarget.11127.

Dorland, Yvonne L., Tsveta S. Malinova, Anne-Marieke D. van Stalborch, Adam G. Grieve, Daphne van Geemen, Nicolette S. Jansen, Bart-Jan de Kreuk, et al. 2016. “The F-BAR Protein pacsin2 Inhibits Asymmetric VE-Cadherin Internalization from Tensile Adherens Junctions.” Nature Communications 7 (July): 12210. oi:10.1038/ncomms12210.

Elfick, Alistair, Grigore Rischitor, Rabah Mouras, Asim Azfer, Lisa Lungaro, Marc Uhlarz, Thomas Herrmannsdörfer, et al. 2017. “Biosynthesis of Magnetic Nanoparticles by Human Mesenchymal Stem Cells Following Transfection with the Magnetotactic Bacterial Gene mms6.” Scientific Reports 7 (January): 39755. doi:10.1038/srep39755.

Faura Tellez, Grissel, Brigitte W. M. Willemse, Uilke Brouwer, Susan Nijboer-Brinksma, Karl Vandepoele, Jacobien A. Noordhoek, Irene Heijink, et al. 2016. “Protocadherin-1 Localization and Cell-Adhesion Function in Airway Epithelial Cells in Asthma.” Edited by Michael Koval. PLOS ONE 11 (10): e0163967. doi:10.1371/journal.pone.0163967.

Garrett, Joshua P., Anthony M. Lowery, Alejandro P. Adam, Andrew P. Kowalczyk, and Peter A. Vincent. 2017. “Regulation of Endothelial Barrier Function by p120-catenin∙VE-Cadherin Interaction.” Edited by Alpha Yap. Molecular Biology of the Cell 28 (1): 85–97. doi:10.1091/mbc.E16-08-0616.

Gillrie, Mark R., Bernard Renaux, Eleanor Russell-Goldman, Marion Avril, Andrew J. Brazier, Koichiro Mihara, Enrico Di Cera, et al. 2016. “Thrombin Cleavage of Plasmodium Falciparum Erythrocyte Membrane Protein 1 Inhibits Cytoadherence.” mBio 7 (5): e01120-16. doi:10.1128/mBio.01120-16.

Gong, Haixia, Menglin Liu, Jeff Klomp, Bradley J. Merrill, Jalees Rehman, and Asrar B. Malik. 2017. “Method for Dual Viral Vector Mediated CRISPR-Cas9 Gene Disruption in Primary Human Endothelial Cells.” Scientific Reports 7 (February): 42127. doi:10.1038/srep42127.

Haines, Ricci J., Richard S. Beard, Rebecca A. Eitner, Liwei Chen, and Mack H. Wu. 2016. “TNFα/IFNγ Mediated Intestinal Epithelial Barrier Dysfunction Is Attenuated by MicroRNA-93 Downregulation of PTK6 in Mouse Colonic Epithelial Cells.” Edited by Jörn Karhausen. PLOS ONE 11 (4): e0154351. doi:10.1371/journal.pone.0154351.

Hong, Jian, Ming Chu, Lijun Qian, Junhong Wang, Yan Guo, and Di Xu. 2017. “Fibrillar Type I Collagen Enhances the Differentiation and Proliferation of Myofibroblasts by Lowering α 2 β 1 Integrin Expression in Cardiac Fibrosis.” BioMed Research International 2017: 1–11. doi:10.1155/2017/1790808.

Jones, A., J. Griffiths, A. Sanders, S. Owen, F. Ruge, K. Harding, and W. Jiang. 2016. “The Clinical Significance and Impact of interleukin?15 on Keratinocyte Cell Growth and Migration.” International Journal of Molecular Medicine, July. doi:10.3892/ijmm.2016.2687.

Kispert, Shannon E., John Marentette, E. Cristian Campian, T. Scott Isbell, Hannah Kuenzel, and Jane McHowat. 2017. “Cigarette Smoke‐induced Urothelial Cell Damage: Potential Role of Platelet‐activating Factor.” Physiological Reports 5 (5): e13177. doi:10.14814/phy2.13177.

Klei, Linda R., Dong Hu, Robert Panek, Danielle N. Alfano, Rachel E. Bridwell, Kelly M. Bailey, Katherine I. Oravecz-Wilson, et al. 2016. “MALT1 Protease Activation Triggers Acute Disruption of Endothelial Barrier Integrity via CYLD Cleavage.” Cell Reports 17 (1): 221–32. doi:10.1016/j.celrep.2016.08.080.

Kostadinova, Elena, Catherine Chaput, Birgitt Gutbier, Juliane Lippmann, Leif E. Sander, Timothy J. Mitchell, Norbert Suttorp, Martin Witzenrath, and Bastian Opitz. 2016. “NLRP3 Protects Alveolar Barrier Integrity by an Inflammasome-Independent Increase of Epithelial Cell Adherence.” Scientific Reports 6 (1): 30943. doi:10.1038/srep30943.

Kostadinova, Elena, Catherine Chaput, Birgitt Gutbier, Juliane Lippmann, Leif E. Sander, Timothy J. Mitchell, Norbert Suttorp, Martin Witzenrath, and Bastian Opitz. 2016. “NLRP3 Protects Alveolar Barrier Integrity by an Inflammasome-Independent Increase of Epithelial Cell Adherence.” Scientific Reports 6 (1): 30943. doi:10.1038/srep30943.

Kostadinova, Elena, Catherine Chaput, Birgitt Gutbier, Juliane Lippmann, Leif E. Sander, Timothy J. Mitchell, Norbert Suttorp, Martin Witzenrath, and Bastian Opitz. 2016. “NLRP3 Protects Alveolar Barrier Integrity by an Inflammasome-Independent Increase of Epithelial Cell Adherence.” Scientific Reports 6 (1): 30943. doi:10.1038/srep30943.

Kovacs-Kasa, Anita, Boris A. Gorshkov, Kyung-Mi Kim, Sanjiv Kumar, Stephen M. Black, David J. Fulton, Christiana Dimitropoulou, John D. Catravas, and Alexander D. Verin. 2016. “The Protective Role of MLCP-Mediated ERM Dephosphorylation in Endotoxin-Induced Lung Injury in Vitro and in Vivo.” Scientific Reports 6 (1): 39018. doi:10.1038/srep39018.

Li, Jin, Lin Ye, Xiaoshun Shi, Jingyi Chen, Fenglan Feng, Yaoqi Chen, Yiren Xiao, et al. 2016. “Repulsive Guidance Molecule B Inhibits Metastasis and Is Associated with Decreased Mortality in Non-Small Cell Lung Cancer.” Oncotarget, March. doi:10.18632/oncotarget.7463.

Miloudi, Khalil, François Binet, Ariel Wilson, Agustin Cerani, Malika Oubaha, Catherine Menard, Sullivan Henriques, et al. 2016. “Truncated Netrin-1 Contributes to Pathological Vascular Permeability in Diabetic Retinopathy.” Journal of Clinical Investigation 126 (8): 3006–22. doi:10.1172/JCI84767.

Montorsi, Lucia, Filippo Guizzetti, Claudia Alecci, Andrea Caporali, Andrea Martello, Claudio Giacinto Atene, Sandra Parenti, et al. 2016. “Loss of zfp36 Expression in Colorectal Cancer Correlates to Wnt/ SS-Catenin Activity and Enhances Epithelial-to-Mesenchymal Transition through Upregulation of zeb1, sox9 and macc.” Oncotarget, September. doi:10.18632/oncotarget.10828.

Nanes, Benjamin A., Cynthia M. Grimsley-Myers, Chantel M. Cadwell, Brian S. Robinson, Anthony M. Lowery, Peter A. Vincent, Marina Mosunjac, Klaus Früh, and Andrew P. Kowalczyk. 2017. “p120-Catenin Regulates VE-Cadherin Endocytosis and Degradation Induced by the Kaposi Sarcoma–associated Ubiquitin Ligase K5.” Edited by Jeffrey D. Hardin. Molecular Biology of the Cell 28 (1): 30–40. doi:10.1091/mbc.E16-06-0459.

Owen, Sioned, Huishan Zhao, Alwyn Dart, Yamei Wang, Fiona Ruge, Yong Gao, Cong Wei, Yiling Wu, and Wen Jiang. 2016. “Heat Shock Protein 27 Is a Potential Indicator for Response to YangZheng XiaoJi and Chemotherapy Agents in Cancer Cells.” International Journal of Oncology, September. doi:10.3892/ijo.2016.3685.

Poroyko, Valeriy A., Alba Carreras, Abdelnaby Khalyfa, Ahamed A. Khalyfa, Vanessa Leone, Eduard Peris, Isaac Almendros, et al. 2016. “Chronic Sleep Disruption Alters Gut Microbiota, Induces Systemic and Adipose Tissue Inflammation and Insulin Resistance in Mice.” Scientific Reports 6 (1): 35405. doi:10.1038/srep35405.

Retzlaff, Jennifer, Kristina Thamm, Chandra C. Ghosh, Wolfgang Ziegler, Hermann Haller, Samir M. Parikh, and Sascha David. 2017. “Flunarizine Suppresses Endothelial Angiopoietin-2 in a Calcium - Dependent Fashion in Sepsis.” Scientific Reports 7 (March): 44113. doi:10.1038/srep44113.

Rom, Slava, Viviana Zuluaga-Ramirez, Nancy L. Reichenbach, Holly Dykstra, Sachin Gajghate, Pal Pacher, and Yuri Persidsky. 2016. “PARP Inhibition in Leukocytes Diminishes Inflammation via Effects on Integrins/cytoskeleton and Protects the Blood-Brain Barrier.” Journal of Neuroinflammation 13 (1): 254. doi:10.1186/s12974-016-0729-x.

Ryman, Valerie E., Nandakumar Packiriswamy, and Lorraine M. Sordillo. 2016. “Apoptosis of Endothelial Cells by 13-HPODE Contributes to Impairment of Endothelial Barrier Integrity.” Mediators of Inflammation 2016: 1–13. doi:10.1155/2016/9867138.

Samra, Yara A., Heba M. Saleh, Khaled A. Hussein, Nehal M. Elsherbiny, Ahmed S. Ibrahim, Khaled Elmasry, Sadanand Fulzele, et al. 2017. “Adenosine Deaminase-2–Induced Hyperpermeability in Human Retinal Vascular Endothelial Cells Is Suppressed by MicroRNA-146b-3p.” Investigative Opthalmology & Visual Science 58 (2): 933. doi:10.1167/iovs.16-19782.

Skaria, Tom, Julia Burgener, Esther Bachli, and Gabriele Schoedon. 2016. “IL-4 Causes Hyperpermeability of Vascular Endothelial Cells through Wnt5A Signaling.” Edited by Binu Tharakan. PLOS ONE 11 (5): e0156002. doi:10.1371/journal.pone.0156002.

Tasev, Dimitar, Lara S. F. Konijnenberg, Joana Amado-Azevedo, Michiel H. van Wijhe, Pieter Koolwijk, and Victor W. M. van Hinsbergh. 2016. “CD34 Expression Modulates Tube-Forming Capacity and Barrier Properties of Peripheral Blood-Derived Endothelial Colony-Forming Cells (ECFCs).” Angiogenesis 19 (3): 325–38. doi:10.1007/s10456-016-9506-9.

Tóth, Gábor, Árpád Szöőr, László Simon, Yosef Yarden, János Szöllősi, and György Vereb. 2016. “The Combination of Trastuzumab and Pertuzumab Administered at Approved Doses May Delay Development of Trastuzumab Resistance by Additively Enhancing Antibody-Dependent Cell-Mediated Cytotoxicity.” mAbs 8 (7): 1361–70. doi:10.1080/19420862.2016.1204503.

Veréb, Zoltán, Szilárd Póliska, Réka Albert, Ole Kristoffer Olstad, Anita Boratkó, Csilla Csortos, Morten C. Moe, Andrea Facskó, and Goran Petrovski. 2016. “Role of Human Corneal Stroma-Derived Mesenchymal-Like Stem Cells in Corneal Immunity and Wound Healing.” Scientific Reports 6 (1): 26227. doi:10.1038/srep26227.

Wagner, Alixandra, Reem Eldawud, Andrew White, Sushant Agarwal, Todd A. Stueckle, Konstantinos A. Sierros, Yon Rojanasakul, Rakesh K. Gupta, and Cerasela Zoica Dinu. 2017. “Toxicity Evaluations of Nanoclays and Thermally Degraded Byproducts through Spectroscopical and Microscopical Approaches.” Biochimica et Biophysica Acta (BBA) - General Subjects 1861 (1): 3406–15. doi:10.1016/j.bbagen.2016.09.003.

Wang, Haibo, Xiaokun Han, Colin A Bretz, Silke Becker, Deeksha Gambhir, George W Smith, R Jude Samulski, et al. 2016. “Retinal Pigment Epithelial Cell Expression of Active Rap 1a by scAAV2 Inhibits Choroidal Neovascularization.” Molecular Therapy - Methods & Clinical Development 3: 16056. doi:10.1038/mtm.2016.56.

Wu, Zhiyong, Zhiwei Wang, Feifeng Dai, Huagang Liu, Wei Ren, Jinxing Chang, and Bowen Li. 2016. “Dephosphorylation of Y685-VE-Cadherin Involved in Pulmonary Microvascular Endothelial Barrier Injury Induced by Angiotensin II.” Mediators of Inflammation 2016: 1–10. doi:10.1155/2016/8696481.

Xie, Lishi, Eddie T. Chiang, Xiaomin Wu, Gabriel T. Kelly, Prasad Kanteti, Patrick A. Singleton, Sara M. Camp, et al. 2016. “Regulation of Thrombin-Induced Lung Endothelial Cell Barrier Disruption by Protein Kinase C Delta.” Edited by Anna A Birukova. PLOS ONE 11 (7): e0158865. doi:10.1371/journal.pone.0158865.

Yao, Ting, Chen-guang Zhang, Ming-tao Gong, Min Zhang, Lei Wang, and Wei Ding. 2016. “Decorin-Mediated Inhibition of the Migration of U87MG Glioma Cells Involves Activation of Autophagy and Suppression of TGF-β Signaling.” FEBS Open Bio 6 (7): 707–19. doi:10.1002/2211-5463.12076.

Zhang, Xun E., Shaquria P. Adderley, and Jerome W. Breslin. 2016. “Activation of RhoA, but Not Rac1, Mediates Early Stages of S1P-Induced Endothelial Barrier Enhancement.” Edited by Binu Tharakan. PLOS ONE 11 (5): e0155490. oi:10.1371/journal.pone.0155490.

Zhang, Yi, Yan Huang, Anna Cantalupo, Paula S. Azevedo, Mauro Siragusa, Jacek Bielawski, Frank J. Giordano, and Annarita Di Lorenzo. 2016. “Endothelial Nogo-B Regulates Sphingolipid Biosynthesis to Promote Pathological Cardiac Hypertrophy during Chronic Pressure Overload.” JCI Insight 1 (5). doi:10.1172/jci.insight.85484.

 
 
 
 

Old Irish Cell Proverb: A watched cell never proliferates!

ECIS Humor

Need a good laugh? Visit the ECIS Cartoons page of our website to view cartoons by Catherine, our in-house cartoonist, to start your day with a smile.

Are you the creative type? Submit one of your own cartoons; if we post it on our website we will send you a free array!
 
 
Footer-logo