Tip of the Month     
Include a cell-free well when doing ECIS experiments

In addition to the more traditional experimental control well with cells, we also recommend having a cell-free, medium-only, well for many experiments.  The cell-free well has a couple of important uses. 

First, it will record any changes in impedance unrelated to cell behavior - for example, changes in solution conductivity and electrode impedance resulting from evaporation of medium from the wells and variations in incubator temperature and pH.   If data is exported to Excel, one may then subtract these cell-free data from the data from other wells to report impedance changes resulting from the cells alone.

In addition, if one is taking multiple frequency time course data (MFT), the medium-only well will record the AC frequency response of the open electrode over time.  These data are most important to acquire if one wishes to model data to report time course changes in the barrier function (Rb), the difficulty of current to flow beneath the cell layer (alpha) and membrane capacitance.

New Product 

Continuous TEER Measurements of Cell Monolayers in Multiple Wells

The ECIS® 24W Trans Filter Array electrically monitors the barrier function of cells grown in culture upon permeable membrane substrates.  The new transwell device accommodates standard 24 well membrane inserts from a broad range of manufacturers.  The adapter is connected to the ECIS® data acquisition system, allowing up to 24 filters to be followed independently.  Dedicated software presents real-time, continuous measurement of TEER in ohm-cm2.  
ECIS Application Webinars

ECIS application webinars review the topics listed below in 20 to 30 minute, web-based, interactive seminars presented by Applied BioPhysics President and co-founder, Dr. Charles Keese.

All webinars are held at 11:00 am EST. To register for a webinar, please go to: https://appliedbiophysics.webex.com and scroll to the webinar date of interest.  

Cell Invasion / Extravasation Assays – October 18, 2016

Automated Cell Migration – November 1, 2016

Barrier Function Assays – November 15, 2016

Real-time Electroporation and Monitoring – December 6, 2016 
Latest ECIS Software   
The latest version of the software is v1.2.215 available from:

Software Tip:

If you have a multi-frequency (MFT) dataset you can switch between the time and frequency modes by selecting the 'T' and 'F' buttons on the toolbar.  By default the first displayed time point is used for the frequency display.  You can also use the Data Cursor to select any point on the time plot and switching to frequency mode will use the selected time index.  The time selected will be indicated on the title of the frequency graph.  The time scroll bar at the bottom of the graph can also be used to scroll through the time points while in frequency mode.
Tradeshows & Events 

International Vascular Biology Meeting 2016
Oct 30 – Nov 3, 2016
Boston, MA

Dec. 3 - 7, 2016
San Francisco, California

2017 Society of Toxicology (SOT) 56th Annual Meeting
March 12 – 16, 2017
Baltimore, Maryland

Experimental Biology 2017
April 22 – 26, 2017
Chicago, IL

American Thoracic Society 2017
May 19 – 24, 2017
Washington, DC 
ECIS in the news:

Binil Starly and Elizabeth Loboa of North Carolina State University lead a team of researchers using ECIS to study how adipose derived stem cells differentiate into bone tissue and the effects of aging on the process.  The work published in Stem Cells Translational Medicine Nordberg et al 2016 builds on the work of Bagnaninchi and Drummond (PNAS 2011; 108:6462) and shows age related differences in the differentiation of bone cells. The authors developed a new ECIS technique to quantify micromotion, using the Shannon entropy equation to calculate the entropy of the ECIS signal. The article has generated a bit of health news buzz. Summary articles can be read at Science Daily or UPI.
New Publications

A couple dozen new publications have been added to the Applied BioPhysics ECIS publications database.  The following are just a selection of those added.

Stolwijk JA, Zhang X, Gueguinou M, Zhang W, Matrougui K, Renken C, et al. Calcium Signaling is Dispensable for Receptor-Regulation of Endothelial Barrier Function. J Biol Chem [Internet]. 2016 Sep 13;jbc.M116.756114. Available from: http://www.jbc.org/lookup/doi/10.1074/jbc.M116.756114

Shaver CM, Upchurch CP, Janz DR, Grove BS, Putz ND, Wickersham NE, et al. Cell-free hemoglobin: a novel mediator of acute lung injury. Am J Physiol - Lung Cell Mol Physiol [Internet]. 2016;310(6):L532–41. Available from: http://ajplung.physiology.org/lookup/doi/10.1152/ajplung.00155.2015

Canning P, Kenny B-A, Prise V, Glenn J, Sarker MH, Hudson N, et al. Lipoprotein-associated phospholipase A2 (Lp-PLA2) as a therapeutic target to prevent retinal vasopermeability during diabetes. Proc Natl Acad Sci U S A [Internet]. 2016 Jun 28 [cited 2016 Sep 27];113(26):7213–8. Available from: http://www.pnas.org/cgi/content/long/113/26/7213

Monickaraj F, McGuire PG, Nitta CF, Ghosh K, Das A. Cathepsin D: an M -derived factor mediating increased endothelial cell permeability with implications for alteration of the blood-retinal barrier in diabetic retinopathy. FASEB J [Internet]. 2016 Apr 1;30(4):1670–82. Available from: http://www.fasebj.org/cgi/doi/10.1096/fj.15-279802

Jensen MR, Bajic G, Zhang X, Laustsen AK, Koldsø H, Skeby KK, et al. Structural Basis for Simvastatin Competitive Antagonism of Complement Receptor 3. J Biol Chem [Internet]. 2016 Aug 12;291(33):16963–76. Available from: http://www.jbc.org/lookup/doi/10.1074/jbc.M116.732222

Chatterjee I, Baruah J, Lurie EE, Wary KK. Endothelial lipid phosphate phosphatase-3 deficiency that disrupts the endothelial barrier function is a modifier of cardiovascular development. Cardiovasc Res [Internet]. 2016 Jul 1;111(1):105–18. Available from: http://cardiovascres.oxfordjournals.org/lookup/doi/10.1093/cvr/cvw090

Jian M-Y, Liu Y, Li Q, Wolkowicz P, Alexeyev M, Zmijewski J, et al. N-cadherin coordinates AMP kinase-mediated lung vascular repair. Am J Physiol - Lung Cell Mol Physiol [Internet]. 2016;310(1):L71–85. Available from: http://ajplung.physiology.org/lookup/doi/10.1152/ajplung.00227.2015

Reinhard NR, van Helden SF, Anthony EC, Yin T, Wu YI, Goedhart J, et al. Spatiotemporal analysis of RhoA/B/C activation in primary human endothelial cells. Sci Rep [Internet]. 2016 May 5;6:25502. Available from: http://www.nature.com/articles/srep25502

Tauseef M, Farazuddin M, Sukriti S, Rajput C, Meyer JO, Ramasamy SK, et al. Transient receptor potential channel 1 maintains adherens junction plasticity by suppressing sphingosine kinase 1 expression to induce endothelial hyperpermeability. FASEB J [Internet]. 2016;30(1):102–10. Available from: http://www.fasebj.org/cgi/doi/10.1096/fj.15-275891

Haasdijk RA, Den Dekker WK, Cheng C, Tempel D, Szulcek R, Bos FL, et al. THSD1 preserves vascular integrity and protects against intraplaque haemorrhaging in ApoE −/− mice. Cardiovasc Res [Internet]. 2016 May 1;110(1):129–39. Available from: http://cardiovascres.oxfordjournals.org/lookup/doi/10.1093/cvr/cvw015

Marcos-Ramiro B, García-Weber D, Barroso S, Feito J, Ortega MC, Cernuda-Morollón E, et al. RhoB controls endothelial barrier recovery by inhibiting Rac1 trafficking to the cell border. J Cell Biol [Internet]. 2016 May 9;213(3):385–402. Available from: http://www.jcb.org/lookup/doi/10.1083/jcb.201504038

Thompson LC, Holland NA, Snyder RJ, Luo B, Becak DP, Odom JT, et al. Pulmonary instillation of MWCNT increases lung permeability, decreases gp130 expression in the lungs, and initiates cardiovascular IL-6 transsignaling. Am J Physiol - Lung Cell Mol Physiol [Internet]. 2016;310(2):L142–54. Available from: http://ajplung.physiology.org/lookup/doi/10.1152/ajplung.00384.2014

ECIS Humor

Need a good laugh? Visit the ECIS Cartoons page of our website to view cartoons by Catherine, our in-house cartoonist, to start your day with a smile.

Are you the creative type? Submit one of your own cartoons; if we post it on our website we will send you a free array!